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Free and esterified carnitine-colorimetric method

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The TAC was measured in tissue homogenates (centrifuged at 15,000 g, 4 C, 10 min) using a kit (MAK187, SigmaAldrich) and a microplate reader (570 nm), as described in our previous study 24

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Subsequently, since the 1920s, it was observed that an adverse side effect of 8-aminoquinolines (primaquine and plasmoquine), used for the treatment and the prophylaxis of malaria, was AHA